产品描述
This Human AQP4 ELISA Kit was designed for the quantitative measurement of Human AQP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
AQP 4 ELISA Kit; AQP-4 ELISA Kit; AQP4 ELISA Kit; AQP4_HUMAN ELISA Kit; Aquaporin type 4 ELISA Kit; Aquaporin-4 ELISA Kit; Aquaporin4 ELISA Kit; HMIWC 2 ELISA Kit; HMIWC2 ELISA Kit; Mercurial insensitive water channel ELISA Kit; Mercurial-insensitive water channel ELISA Kit; MGC22454 ELISA Kit; MIWC ELISA Kit; WCH 4 ELISA Kit; WCH4 ELISA Kit
缩写
AQP4
Uniprot No
P55087
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 92-103 | |
| 1:2 | Average % | 87 | |
| Range % | 82-93 | |
| 1:4 | Average % | 92 | |
| Range % | 87-96 | |
| 1:8 | Average % | 90 | |
| Range % | 88-93 | |
回收率
| The recovery of human AQP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 90-106 | |
| EDTA plasma (n=4) | 92 | 85-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.568 | 2.555 | 2.562 | 2.472 | | | 5 | 1.725 | 1.678 | 1.702 | 1.612 | | | 2.5 | 1.113 | 1.101 | 1.107 | 1.017 | | | 1.25 | 0.647 | 0.639 | 0.643 | 0.553 | | | 0.625 | 0.381 | 0.356 | 0.369 | 0.279 | | | 0.312 | 0.218 | 0.209 | 0.214 | 0.124 | | | 0.156 | 0.147 | 0.137 | 0.142 | 0.052 | | | 0 | 0.092 | 0.088 | 0.090 | | |
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货期
3-5 working days