产品描述
This Rat CDH1 ELISA Kit was designed for the quantitative measurement of Rat CDH1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit
缩写
CDH1
Uniprot No
Q9R0T4
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 85-90 | | |
| 1:2 | Average % | 102 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 99 | | |
| Range % | 95-103 | | |
| 1:8 | Average % | 83 | | |
| Range % | 80-86 | | |
回收率
| The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 105 | 101-109 | | |
| EDTA plasma (n=4) | 97 | 93-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.750 | 2.798 | 2.774 | 2.591 | | | | 100 | 2.045 | 1.968 | 2.007 | 1.824 | | | | 50 | 1.401 | 1.358 | 1.380 | 1.197 | | | | 25 | 0.920 | 0.967 | 0.944 | 0.761 | | | | 12.5 | 0.576 | 0.598 | 0.587 | 0.404 | | | | 6.25 | 0.445 | 0.468 | 0.457 | 0.274 | | | | 3.12 | 0.307 | 0.312 | 0.310 | 0.127 | | | | 0 | 0.182 | 0.183 | 0.183 | | | |
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货期
3-5 working days