产品描述
CRP,即C-反应蛋白(C-reactive protein),是一种急性相蛋白,主要由肝脏合成,可作为炎症的非特异性生物标志物。当机体遇到感染、损伤、炎症等刺激时,肝脏合成和分泌CRP,其浓度会在数小时内显著升高。因此,CRP常被用于评估感染、炎症和自身免疫性疾病等疾病的活动程度和预后,也可用于评估心血管疾病和癌症等疾病的风险。正常情况下,血浆中CRP的浓度很低,但在炎症反应期可升高数千倍。
华美生物所提供的Rat C-Reactive Protein,CRP ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆、细胞裂解液样本中的CRP,其灵敏度为7.81 ng/mL,检测范围为31.25 ng/mL-2000 ng/mL。
别名
Crp ELISA kit; Ptx1C-reactive protein ELISA kit
缩写
CRP
Uniprot No
P48199
灵敏度
7.81 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
92-105 |
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回收率
| The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
88-97 |
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| EDTA plasma (n=4) |
96 |
91-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.664 |
2.701 |
2.683 |
2.541 |
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| 1000 |
1.701 |
1.716 |
1.709 |
1.567 |
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| 500 |
1.173 |
1.182 |
1.178 |
1.036 |
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| 250 |
0.604 |
0.589 |
0.597 |
0.455 |
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| 125 |
0.398 |
0.372 |
0.385 |
0.243 |
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| 62.5 |
0.302 |
0.288 |
0.295 |
0.153 |
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| 31.25 |
0.234 |
0.227 |
0.231 |
0.089 |
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| 0 |
0.144 |
0.140 |
0.142 |
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货期
3-5 working days