产品描述
CCL24,也称为炎性细胞趋化蛋白-2(MIP-2),是C-C趋化因子家族中的一种趋化因子。它主要由树突状细胞、纤维细胞和肌肉细胞等产生,并且能够吸引CCR3受体表达的细胞,如嗜酸性粒细胞和嗜碱性粒细胞等。CCL24在过敏性疾病、哮喘和皮肤炎症等多种疾病中发挥着重要作用。
华美生物所提供的Human Eotaxin 2/CCL24 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CCL24,其灵敏度为67.660 pg/mL,检测范围为78.125 pg/mL-5000 pg/mL。
别名
C C motif chemokine 24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; CCL24 ELISA Kit; CCL24_HUMAN ELISA Kit; Chemokine CC Motif Ligand 24 ELISA Kit; CK beta 6 ELISA Kit; CK-beta-6 ELISA Kit; Ckb6 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; MPIF 2 ELISA Kit; MPIF-2 ELISA Kit; MPIF2 ELISA Kit; Myeloid progenitor inhibitory factor 2 ELISA Kit; SCYA24 ELISA Kit; Small inducible cytokine A24 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys) member 24 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
缩写
CCL24
Uniprot No
O00175
灵敏度
67.660 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-94 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-108 |
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回收率
| The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-103 |
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| EDTA plasma (n=4) |
86 |
83-89 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.152 |
2.204 |
2.178 |
2.065 |
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| 2500 |
1.796 |
1.735 |
1.766 |
1.653 |
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| 1250 |
1.182 |
1.205 |
1.194 |
1.081 |
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| 625 |
0.765 |
0.798 |
0.782 |
0.669 |
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| 312.5 |
0.453 |
0.468 |
0.461 |
0.348 |
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| 156.25 |
0.328 |
0.331 |
0.330 |
0.217 |
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| 78.125 |
0.251 |
0.240 |
0.246 |
0.133 |
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| 0 |
0.115 |
0.110 |
0.113 |
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货期
3-5 working days