产品描述
This Human AQP-4 Ab ELISA Kit was designed for the quantitative measurement of Human AQP-4 Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
缩写
AQP-4 Ab
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
84-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:8 |
Average % |
91 |
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| Range % |
82-97 |
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回收率
| The recovery of human AQP-4 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
82-197 |
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| EDTA plasma (n=4) |
95 |
84-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.615 |
2.575 |
2.595 |
2.456 |
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| 50 |
1.988 |
2.103 |
2.046 |
1.907 |
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| 25 |
1.299 |
1.312 |
1.306 |
1.167 |
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| 12.5 |
0.672 |
0.675 |
0.674 |
0.535 |
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| 6.25 |
0.407 |
0.392 |
0.400 |
0.261 |
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| 3.12 |
0.294 |
0.278 |
0.286 |
0.147 |
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| 1.56 |
0.225 |
0.214 |
0.220 |
0.081 |
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| 0 |
0.142 |
0.135 |
0.139 |
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货期
3-5 working days