产品描述
This Human APOC2 ELISA Kit was designed for the quantitative measurement of Human APOC2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.2 ng/mL-2000 ng/mL and the sensitivity is 15.6 ng/mL.
别名
APC 2 ELISA Kit; APC2 ELISA Kit; Apo CII ELISA Kit; Apo-CII ELISA Kit; APOC 2 ELISA Kit; ApoC II ELISA Kit; ApoC-II ELISA Kit; APOC2 ELISA Kit; APOC2 protein ELISA Kit; APOC2_HUMAN ELISA Kit; ApoCII ELISA Kit; Apolipoprotein C II ELISA Kit; Apolipoprotein C II precursor ELISA Kit; Apolipoprotein C2 ELISA Kit; ApolipoproteinCII ELISA Kit; MGC75082 ELISA Kit; ProapoC-II ELISA Kit; Proapolipoprotein C-II ELISA Kit
缩写
APOC2
Uniprot No
P02655
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:100 | Average % | 98 | |
| Range % | 90-102 | |
| 1:200 | Average % | 88 | |
| Range % | 82-92 | |
| 1:400 | Average % | 100 | |
| Range % | 90-104 | |
| 1:800 | Average % | 101 | |
| Range % | 91-105 | |
回收率
| The recovery of human apo-C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 90-102 | |
| EDTA plasma (n=4) | 92 | 88-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | | | | 2000 | 0.088 | 0.093 | 0.091 | | | | 1000 | 0.215 | 0.221 | 0.218 | | | | 500 | 0.415 | 0.424 | 0.420 | | | | 250 | 0.693 | 0.682 | 0.688 | | | | 125 | 1.095 | 1.077 | 1.086 | | | | 62.5 | 1.521 | 1.500 | 1.511 | | | | 31.2 | 1.668 | 1.598 | 1.633 | | | | 0 | 2.034 | 1.987 | 2.011 | | |
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货期
3-5 working days