产品描述
This Human Col Ⅱ ELISA Kit was designed for the quantitative measurement of Human Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
Collagen Type 2,Col 2,_x000D_
Collagen Type II,Col II
缩写
Col Ⅱ
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
86 |
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| Range % |
80-90 |
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| 1:200 |
Average % |
93 |
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| Range % |
88-98 |
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| 1:400 |
Average % |
94 |
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| Range % |
90-100 |
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| 1:800 |
Average % |
96 |
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| Range % |
90-102 |
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回收率
| The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-98 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.478 |
2.387 |
2.433 |
2.341 |
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| 1000 |
1.811 |
1.752 |
1.782 |
1.690 |
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| 500 |
1.237 |
1.142 |
1.190 |
1.098 |
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| 250 |
0.863 |
0.813 |
0.838 |
0.746 |
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| 125 |
0.398 |
0.374 |
0.386 |
0.294 |
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| 62.5 |
0.211 |
0.198 |
0.205 |
0.113 |
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| 31.25 |
0.145 |
0.141 |
0.143 |
0.051 |
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| 0 |
0.093 |
0.090 |
0.092 |
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货期
3-5 working days