产品描述
This Mouse COL18A1/ES ELISA Kit was designed for the quantitative measurement of Mouse COL18A1/ES protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
Col18a1Collagen alpha-1(XVIII) chain [Cleaved into: Endostatin; Non-collagenous domain 1 ELISA Kit; NC1)] ELISA Kit
缩写
COL18A1/ES
Uniprot No
P39061
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL18A1/ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-95 |
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| 1:2 |
Average % |
103 |
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| Range % |
99-105 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
99 |
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| Range % |
94-101 |
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回收率
| The recovery of mouse COL18A1/ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-98 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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标准曲线
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.234 |
2.213 |
2.224 |
2.067 |
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| 5 |
1.732 |
1.694 |
1.713 |
1.556 |
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| 2.5 |
1.091 |
1.076 |
1.084 |
0.927 |
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| 1.25 |
0.551 |
0.537 |
0.544 |
0.387 |
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| 0.625 |
0.366 |
0.386 |
0.376 |
0.219 |
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| 0.312 |
0.292 |
0.281 |
0.287 |
0.130 |
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| 0.156 |
0.248 |
0.228 |
0.238 |
0.081 |
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| 0 |
0.159 |
0.155 |
0.157 |
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货期
3-5 working days