别名
2B4 ELISA Kit; C9.1 ELISA Kit; CD244 ELISA Kit; CD244 antigen ELISA Kit; CD244 molecule ELISA Kit; CD244 molecule natural killer cell receptor 2B4 ELISA Kit; CD244 natural killer cell receptor 2B4 ELISA Kit; CD244_HUMAN ELISA Kit; F730046O15Rik ELISA Kit; h2B4 ELISA Kit; Ly90 ELISA Kit; NAIL ELISA Kit; Natural killer cell activation-inducing ligand ELISA Kit; Natural killer cell receptor 2B4 ELISA Kit; NK cell activation inducing ligand ELISA Kit; NK cell activation inducing ligand NAIL ELISA Kit; NK cell activation-inducing ligand ELISA Kit; NK cell type I receptor protein 2B4 ELISA Kit; NKR2B4 ELISA Kit; Nmrk ELISA Kit; Non-MHC restricted killing associated ELISA Kit; OTTHUMP00000027884 ELISA Kit; p38 ELISA Kit; signaling lymphocytic activation molecule 4 ELISA Kit; SLAM family, member 4 ELISA Kit; SLAMF4 ELISA Kit
缩写
CD244
Uniprot No
Q9BZW8
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD244 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 88-96 | | |
| 1:2 | Average % | 102 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 99 | | |
| Range % | 95-103 | | |
| 1:8 | Average % | 89 | | |
| Range % | 85-93 | | |
回收率
| The recovery of human CD244 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 104 | 100-108 | | |
| EDTA plasma (n=4) | 97 | 93-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 40 | 2.173 | 2.200 | 2.187 | 2.049 | | | | 20 | 1.604 | 1.700 | 1.652 | 1.514 | | | | 10 | 1.118 | 1.204 | 1.161 | 1.023 | | | | 5 | 0.765 | 0.750 | 0.758 | 0.620 | | | | 2.5 | 0.503 | 0.498 | 0.501 | 0.363 | | | | 1.25 | 0.335 | 0.341 | 0.338 | 0.200 | | | | 0.625 | 0.227 | 0.231 | 0.229 | 0.091 | | | | 0 | 0.139 | 0.136 | 0.138 | | | |
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货期
3-5 working days