产品描述
This Human Col Ⅳ ELISA Kit was designed for the quantitative measurement of Human Col Ⅳ protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .
别名
Collagen Type 4(COL 4),_x000D_
Collagen Type IV(COL IV)
缩写
Col Ⅳ
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
86 |
|
| Range % |
80-104 |
|
| 1:400 |
Average % |
97 |
|
| Range % |
91-105 |
|
| 1:800 |
Average % |
100 |
|
| Range % |
92-113 |
|
| 1:1600 |
Average % |
93 |
|
| Range % |
86-98 |
|
回收率
| The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
89-102 |
|
| EDTA plasma (n=4) |
93 |
85-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 4000 |
2.748 |
2.856 |
2.802 |
2.635 |
|
| 2000 |
2.459 |
2.436 |
2.448 |
2.281 |
|
| 1000 |
1.876 |
1.968 |
1.922 |
1.755 |
|
| 500 |
1.240 |
1.234 |
1.237 |
1.070 |
|
| 250 |
0.843 |
0.836 |
0.840 |
0.673 |
|
| 125 |
0.434 |
0.447 |
0.441 |
0.274 |
|
| 62.5 |
0.246 |
0.256 |
0.251 |
0.084 |
|
| 0 |
0.165 |
0.168 |
0.167 |
|
|
|
货期
3-5 working days