产品描述
CD163是一种膜上糖蛋白,属于血红素结合蛋白家族,在巨噬细胞和单核细胞等免疫细胞中表达。它主要作为针对血红素的清除受体,参与细胞内的血红素代谢、调节炎症反应和调节免疫应答等过程。
华美生物所提供的Human soluble CD163(sCD163) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CD163,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
C163A_HUMAN ELISA Kit; CD 163 ELISA Kit; CD163 ELISA Kit; CD163 antigen ELISA Kit; CD163 molecule ELISA Kit; Hemoglobin scavenger receptor ELISA Kit; M130 ELISA Kit; M130 antigen precursor ELISA Kit; Macrophage associated antigen ELISA Kit; MM130 ELISA Kit; OTTHUMP00000238617 ELISA Kit; OTTHUMP00000238618 ELISA Kit; OTTHUMP00000238619 ELISA Kit; OTTHUMP00000238620 ELISA Kit; SCARI1 ELISA Kit; Scavenger receptor cysteine rich type 1 protein M130 ELISA Kit; sCD163 ELISA Kit; Soluble CD163 ELISA Kit
缩写
CD163
Uniprot No
Q86VB7
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
88 |
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| Range % |
83-93 |
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| 1:10 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:20 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:40 |
Average % |
95 |
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| Range % |
91-99 |
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回收率
| The recovery of human sCD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
98 |
93-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.452 |
2.552 |
2.502 |
2.368 |
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| 50 |
2.134 |
2.176 |
2.155 |
2.021 |
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| 25 |
1.539 |
1.497 |
1.518 |
1.384 |
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| 12.5 |
0.728 |
0.739 |
0.734 |
0.600 |
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| 6.25 |
0.412 |
0.424 |
0.418 |
0.284 |
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| 3.12 |
0.229 |
0.239 |
0.234 |
0.100 |
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| 1.56 |
0.194 |
0.201 |
0.198 |
0.064 |
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| 0 |
0.132 |
0.135 |
0.134 |
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货期
3-5 working days