产品描述
This Mouse BMP6 ELISA Kit was designed for the quantitative measurement of Mouse BMP6 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
别名
Bmp6 ELISA kit; Bmp-6 ELISA kit; Vgr1Bone morphogenetic protein 6 ELISA kit; BMP-6 ELISA kit; VG-1-related protein ELISA kit; VGR-1 ELISA kit
缩写
BMP6
Uniprot No
P20722
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-102 | |
| 1:2 | Average % | 96 | |
| Range % | 82-101 | |
| 1:4 | Average % | 98 | |
| Range % | 93-107 | |
| 1:8 | Average % | 97 | |
| Range % | 92-105 | |
回收率
| The recovery of mouse BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 100 | 95-105 | |
| EDTA plasma (n=4) | 102 | 96-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.616 | 2.582 | 2.599 | 2.414 | | | 1000 | 2.215 | 2.234 | 2.225 | 2.040 | | | 500 | 1.801 | 1.834 | 1.818 | 1.633 | | | 250 | 1.367 | 1.330 | 1.349 | 1.164 | | | 125 | 0.768 | 0.743 | 0.756 | 0.571 | | | 62.5 | 0.469 | 0.442 | 0.456 | 0.271 | | | 31.25 | 0.272 | 0.292 | 0.282 | 0.097 | | | 0 | 0.184 | 0.186 | 0.185 | | |
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货期
3-5 working days