产品描述

This human CTRP3 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CTRP3 in multiple samples, including serum, plasma, or cell culture supernates. Antibody specific for CTRP3 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any CTRP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated CTRP3 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of CTRP3 bound in the initial step.

CTRP3 (C1QTNF3) is one of the anti-inflammatory adipocytokines and inversely correlates with pro-inflammatory cytokines such as IL-6, TNF, and CRP. It is involved in various physiological and pathological processes, including glucose/lipid metabolism, inflammation, proliferation, apoptosis, vascular calcification, fibrosis, ischemic injury, and cardiovascular protection. CTRP3 also influences some specific inflammatory disorders, such as immunoglobulin A nephropathy and inflammatory bowel disease. Apart from its role in inflammation, CTRP3 affects the differentiation and adipogenesis of adipocytes.

别名

C1ATNF3 ELISA Kit; C1q and tumor necrosis factor related protein 3 ELISA Kit; C1QT3_HUMAN ELISA Kit; C1qtnf3 ELISA Kit; Cartonectin ELISA Kit; Collagenous repeat containing sequence of 26 kDa ELISA Kit; collagenous repeat-containing sequence 26 kDa protein ELISA Kit; Complement C1q tumor necrosis factor related protein 3 ELISA Kit; Complement C1q tumor necrosis factor-related protein 3 ELISA Kit; Corcs ELISA Kit; CORS 26 ELISA Kit; Cors ELISA Kit; CORS26 ELISA Kit; CTRP3 ELISA Kit; FLJ37576 ELISA Kit; OTTHUMP00000115918 ELISA Kit; OTTHUMP00000219981 ELISA Kit; PRO1484 ELISA Kit; Secretory protein CORS26 ELISA Kit; UNQ753 ELISA Kit

缩写

C1QTNF3

Uniprot No

Q9BXJ4

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CTRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		81-95
1:2		Average %		92
		Range %		90-102
1:4		Average %		96
		Range %		91-100
1:8		Average %		95
		Range %		89-102

回收率

The recovery of human CTRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		85-100
EDTA plasma (n=4)		101		90-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 1.839 1.952 1.896 1.741 5 1.616 1.586 1.601 1.446 2.5 1.354 1.271 1.313 1.158 1.25 0.912 0.932 0.922 0.767 0.625 0.617 0.609 0.613 0.458 0.312 0.412 0.388 0.400 0.245 0.156 0.263 0.251 0.257 0.102 0 0.159 0.151 0.155

货期

3-5 working days