产品描述
This Pig cTn-I ELISA Kit was designed for the quantitative measurement of Pig cTn-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
缩写
cTnⅠ
Uniprot No
I3LLI0
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
89-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
83-99 |
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回收率
| The recovery of pig cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-101 |
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| EDTA plasma (n=4) |
95 |
86-106 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
1.815 |
1.866 |
1.841 |
1.703 |
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| 500 |
1.652 |
1.713 |
1.683 |
1.545 |
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| 250 |
1.347 |
1.401 |
1.374 |
1.236 |
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| 125 |
1.006 |
0.957 |
0.982 |
0.844 |
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| 62.5 |
0.743 |
0.725 |
0.734 |
0.596 |
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| 31.25 |
0.514 |
0.508 |
0.511 |
0.373 |
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| 15.6 |
0.324 |
0.308 |
0.316 |
0.178 |
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| 0 |
0.136 |
0.139 |
0.138 |
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货期
3-5 working days