产品描述
血管紧张素转化酶(ACE)是一种重要的生物酶,它可以帮助人体控制血压。ACE有两个主要的功能:一是将血管紧张素I转化成血管紧张素II,二是抑制血小板聚集。它可以通过将血管紧张素I转化为血管紧张素II来改变血压水平。 ACE也可以帮助人体抵御感染,因为它能够促进免疫应答的形成。
华美生物所提供的Mouse Angiotensin converting enzyme,ACE ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的ACE,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
Ace ELISA kit; Dcp1Angiotensin-converting enzyme ELISA kit; ACE ELISA kit; EC 3.2.1.- ELISA kit; EC 3.4.15.1 ELISA kit; Dipeptidyl carboxypeptidase I ELISA kit; Kininase II ELISA kit; CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme ELISA kit; soluble form] ELISA kit
缩写
ACE
Uniprot No
P09470
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
95 |
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| Range % |
84-101 |
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| 1:10 |
Average % |
96 |
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| Range % |
86-103 |
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| 1:20 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:40 |
Average % |
95 |
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| Range % |
87-101 |
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回收率
| The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-103 |
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| EDTA plasma (n=4) |
92 |
85-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.153 |
2.141 |
2.147 |
1.971 |
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| 50 |
1.542 |
1.596 |
1.569 |
1.393 |
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| 25 |
1.117 |
1.104 |
1.111 |
0.935 |
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| 12.5 |
0.703 |
0.688 |
0.696 |
0.520 |
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| 6.25 |
0.489 |
0.474 |
0.482 |
0.306 |
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| 3.12 |
0.322 |
0.318 |
0.320 |
0.144 |
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| 1.56 |
0.259 |
0.266 |
0.263 |
0.087 |
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| 0 |
0.172 |
0.180 |
0.176 |
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货期
3-5 working days