产品描述
This Rat CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Rat CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
C-telopeptide of type 2 collagen,CTX-2,_x000D_
C-telopeptide of type II collagen,CTX-II
缩写
CTX-Ⅱ
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-104 |
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| 1:4 |
Average % |
91 |
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| Range % |
87-94 |
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| 1:8 |
Average % |
98 |
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| Range % |
95-103 |
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回收率
| The recovery of rat CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
91-98 |
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| EDTA plasma (n=4) |
95 |
91-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.232 |
2.202 |
2.217 |
2.076 |
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| 10 |
1.458 |
1.447 |
1.453 |
1.312 |
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| 5 |
0.888 |
0.900 |
0.894 |
0.753 |
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| 2.5 |
0.635 |
0.621 |
0.628 |
0.487 |
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| 1.25 |
0.375 |
0.362 |
0.369 |
0.228 |
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| 0.625 |
0.294 |
0.288 |
0.291 |
0.150 |
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| 0.312 |
0.238 |
0.227 |
0.233 |
0.092 |
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| 0 |
0.142 |
0.140 |
0.141 |
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货期
3-5 working days