产品描述
This Mouse ANA Ab (IgG) ELISA Kit was designed for the quantitative measurement of Mouse ANA Ab (IgG) protein in serum, plasma. It is a Capture ELISA ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
缩写
ANA Ab (IgG)
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
85 |
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| Range % |
82-89 |
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| 1:800 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.751 |
2.752 |
2.752 |
2.559 |
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| 250 |
2.327 |
2.377 |
2.352 |
2.159 |
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| 125 |
1.700 |
1.675 |
1.688 |
1.495 |
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| 62.5 |
0.993 |
0.968 |
0.981 |
0.788 |
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| 31.2 |
0.637 |
0.625 |
0.631 |
0.438 |
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| 15.6 |
0.401 |
0.416 |
0.409 |
0.216 |
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| 7.8 |
0.299 |
0.289 |
0.294 |
0.101 |
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| 0 |
0.188 |
0.197 |
0.193 |
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货期
3-5 working days