产品描述
The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is your go-to solution for accurate and reliable quantification of Beta-2-microglobulin in rat serum, plasma, urine, and tissue homogenates.
Designed for researchers in the field of immunology、autoimmune diseases and renal function research, this ELISA kit provides a quantitative measurement of B2M levels with a detection range of 0.156 μg/mL-10 μg/mL and a sensitivity of 0.039 μg/mL, ensuring precise and reproducible results.
The assay principle is based on a sandwich measurement, where the captured rat β2-microglobulin is detected using a specific antibody conjugated to horseradish peroxidase. The kit's assay time is 1-5 hours, and the sample volume required is between 50-100ul.
The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is compatible with standard 96-well microplates and requires a detection wavelength of 450 nm. This ELISA kit has been cited in more than 3 scientific publications, demonstrating its robust performance and reliability.
别名
B2MBeta-2-microglobulin ELISA kit
缩写
B2M
Uniprot No
P01885
灵敏度
0.039 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-92 | |
| 1:2 | Average % | 96 | |
| Range % | 92-101 | |
| 1:4 | Average % | 100 | |
| Range % | 95-105 | |
| 1:8 | Average % | 90 | |
| Range % | 85-95 | |
回收率
| The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 90-104 | |
| EDTA plasma (n=4) | 93 | 89-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | μg/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.645 | 2.678 | 2.662 | 2.515 | | | 5 | 1.855 | 1.896 | 1.876 | 1.729 | | | 2.5 | 1.256 | 1.263 | 1.260 | 1.113 | | | 1.25 | 0.834 | 0.869 | 0.852 | 0.705 | | | 0.625 | 0.487 | 0.489 | 0.488 | 0.341 | | | 0.312 | 0.325 | 0.353 | 0.339 | 0.192 | | | 0.156 | 0.261 | 0.284 | 0.273 | 0.126 | | | 0 | 0.145 | 0.149 | 0.147 | | |
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货期
3-5 working days
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