产品描述
This Human APOC3 ELISA Kit was designed for the quantitative measurement of Human APOC3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.
别名
APOC3 ELISA Kit; APO C3 ELISA Kit; Apo CIII ELISA Kit; Apo-CIII ELISA Kit; APOC 3 ELISA Kit; ApoC III ELISA Kit; ApoC-III ELISA Kit; APOC3 ELISA Kit; APOC3_HUMAN ELISA Kit; ApoCIII ELISA Kit; Apolipoprotein C III ELISA Kit; Apolipoprotein C-III ELISA Kit; Apolipoprotein C3 ELISA Kit; ApolipoproteinCIII ELISA Kit; MGC150353 ELISA Kit
缩写
APOC3
Uniprot No
P02656
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
95 |
|
| Range % |
87-99 |
|
| 1:200 |
Average % |
89 |
|
| Range % |
81-94 |
|
| 1:400 |
Average % |
101 |
|
| Range % |
91-105 |
|
| 1:800 |
Average % |
100 |
|
| Range % |
90-105 |
|
回收率
| The recovery of human apo-C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
87-98 |
|
| EDTA plasma (n=4) |
97 |
90-103 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 1000 |
0.080 |
0.085 |
0.083 |
|
|
| 500 |
0.126 |
0.123 |
0.125 |
|
|
| 250 |
0.200 |
0.209 |
0.205 |
|
|
| 125 |
0.363 |
0.370 |
0.367 |
|
|
| 62.5 |
0.587 |
0.612 |
0.600 |
|
|
| 31.25 |
0.804 |
0.823 |
0.814 |
|
|
| 15.6 |
1.112 |
1.164 |
1.138 |
|
|
| 0 |
1.944 |
1.878 |
1.911 |
|
|
|
货期
3-5 working days