产品描述

The Human CASP3 ELISA Kit allows for the in vitro quantitative determination of CASP3 concentrations in serum, plasma, cell lysates, or tissue homogenates. This kit exclusively recognizes human CASP3. CASP3 is a frequently activated death protease and is responsible for the proteolytic cleavage of many specific target proteins leading to typical morphological changes in apoptotic cells. Studies have shown that CASP3 induced apoptosis in neurons during programmed developmental cell death and that CASP3 is required for normal brain development. CASP3-induced apoptosis has also been observed in microglia and astrocytes of developing brains. In addition to its apoptotic functions, CASP3 has also been demonstrated to influence the survival, proliferation, and differentiation of both normal and malignant cells and tissues. CASP3 is also necessary for the development of the auditory function.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. CASP3 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled CASP3 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with CASP3 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

别名

A830040C14Rik ELISA Kit; Apopain ELISA Kit; CASP 3 ELISA Kit; CASP-3 ELISA Kit; CASP3 ELISA Kit; CASP3_HUMAN ELISA Kit; Casp3a ELISA Kit; Caspase 3 ELISA Kit; Caspase 3; apoptosis-related cysteine peptidase ELISA Kit; Caspase 3; apoptosis-related cysteine protease ELISA Kit; Caspase 3; apoptosis-related cysteine protease a ELISA Kit; Caspase-3 subunit p12 ELISA Kit; Caspase3 ELISA Kit; CC3 ELISA Kit; CPP 32 ELISA Kit; CPP-32 ELISA Kit; CPP32 ELISA Kit; CPP32B ELISA Kit; Cysteine protease CPP32 ELISA Kit; EC 3.4.22.56 ELISA Kit; ICE3 ELISA Kit; LICE ELISA Kit; mldy ELISA Kit; OTTHUMP00000165052 ELISA Kit; OTTHUMP00000165053 ELISA Kit; OTTHUMP00000165054 ELISA Kit; PARP cleavage protease ELISA Kit; Procaspase3 ELISA Kit; Protein Yama ELISA Kit; SCA 1 ELISA Kit; SCA-1 ELISA Kit; SCA1 ELISA Kit; SREBP cleavage activity 1 ELISA Kit; Yama ELISA Kit; Yama protein ELISA Kit

缩写

CASP3

Uniprot No

P42574

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-101
1:2		Average %		94
		Range %		83-101
1:4		Average %		96
		Range %		90-106
1:8		Average %		94
		Range %		85-105

回收率

The recovery of human Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-104
EDTA plasma (n=4)		96		91-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.758 2.762 2.760 2.653   10 2.476 2.461 2.469 2.362   5 2.002 1.902 1.952 1.845   2.5 1.245 1.209 1.227 1.120   1.25 0.622 0.612 0.617 0.510   0.625 0.308 0.325 0.317 0.210   0.312 0.212 0.224 0.218 0.111   0 0.105 0.109 0.107

货期

3-5 working days