产品描述
CCL27,也称为皮肤趋化蛋白-3(CTACK),是C-C趋化因子家族中的一种趋化因子。它主要由表皮细胞和角质形成细胞等皮肤细胞产生,并且能够吸引CCR10受体表达的细胞,如Th1和Th2细胞等。CCL27在皮肤免疫应答中发挥着重要作用,如过敏性皮肤炎、银屑病等。
华美生物所提供的Human C-C motif chemokine 27 (CCL27) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CCL27,其灵敏度为0.043 ng/mL,检测范围为0.313 ng/mL-20 ng/mL。
别名
ALP ELISA Kit; C-C motif chemokine 27 ELISA Kit; CC chemokine ILC ELISA Kit; Ccl27 ELISA Kit; CCL27_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 27 ELISA Kit; CTACK ELISA Kit; CTAK ELISA Kit; Cutaneous T cell attracting chemokine ELISA Kit; Cutaneous T-cell-attracting chemokine ELISA Kit; ESkine ELISA Kit; IL 11 R alpha locus chemokine ELISA Kit; IL-11 R-alpha-locus chemokine ELISA Kit; ILC ELISA Kit; PESKY ELISA Kit; SCYA27 ELISA Kit; Skinkine ELISA Kit; Small inducible cytokine A27 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 27 ELISA Kit; Small-inducible cytokine A27 ELISA Kit
缩写
CCL27
Uniprot No
Q9Y4X3
灵敏度
0.043 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCL27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-88 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
104 |
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| Range % |
101-107 |
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| 1:8 |
Average % |
89 |
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| Range % |
85-93 |
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回收率
| The recovery of human CCL27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
94-100 |
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| EDTA plasma (n=4) |
105 |
101-109 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.715 |
2.768 |
2.742 |
2.589 |
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| 10 |
2.097 |
2.014 |
2.056 |
1.903 |
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| 5 |
1.276 |
1.234 |
1.255 |
1.102 |
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| 2.5 |
0.752 |
0.768 |
0.760 |
0.607 |
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| 1.25 |
0.481 |
0.498 |
0.490 |
0.337 |
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| 0.625 |
0.327 |
0.310 |
0.319 |
0.166 |
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| 0.313 |
0.257 |
0.244 |
0.251 |
0.098 |
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| 0 |
0.156 |
0.150 |
0.153 |
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货期
3-5 working days