产品描述
CUSABIO's mouse MCP-3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP-3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of MCP-3 in samples. The color intensity is measured at 450 nm via a microplate reader.
MCP-3, also called CCL7, is a chemotactic factor and attractant for various kinds of leukocytes, including monocytes and neutrophils. CCL7 is involved in anti-inflammatory responses through binding to its chemokine receptors CCR1, CCR2, CCR3, and CCR5 to mediate the recruitment of immune cells. Abnormal CCL7 expression is related to certain immune diseases. Furthermore, CCL7 plays a pivotal role in tumorigenesis. CCL7 promotes tumor progression by supporting the formation of the tumor microenvironment (TME) and facilitating tumor invasion and metastasis.
别名
Ccl7 ELISA kit; Fic ELISA kit; Mcp3 ELISA kit; Scya7C-C motif chemokine 7 ELISA kit; Intercrine/chemokine MARC ELISA kit; Monocyte chemoattractant protein 3 ELISA kit; Monocyte chemotactic protein 3 ELISA kit; MCP-3 ELISA kit; Protein FIC ELISA kit; Small-inducible cytokine A7 ELISA kit
缩写
CCL7
Uniprot No
Q03366
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
86-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
93 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-101 |
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回收率
| The recovery of mouse MCP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
84-92 |
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| EDTA plasma (n=4) |
100 |
91-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.610 |
2.500 |
2.555 |
2.448 |
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| 50 |
2.197 |
2.125 |
2.161 |
2.054 |
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| 25 |
1.451 |
1.466 |
1.459 |
1.352 |
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| 12.5 |
0.750 |
0.776 |
0.763 |
0.656 |
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| 6.25 |
0.346 |
0.340 |
0.343 |
0.236 |
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| 3.12 |
0.240 |
0.233 |
0.237 |
0.130 |
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| 1.56 |
0.168 |
0.159 |
0.164 |
0.057 |
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| 0 |
0.106 |
0.108 |
0.107 |
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货期
3-5 working days
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