产品描述
This Human Col Ⅲ ELISA Kit was designed for the quantitative measurement of Human Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL .
别名
Collagen Type 3,Col 3,_x000D_
Collagen Type III,Col III
缩写
Col Ⅲ
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-96 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
92 |
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| Range % |
85-98 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-102 |
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回收率
| The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
80-94 |
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| EDTA plasma (n=4) |
97 |
92-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.850 |
2.963 |
2.907 |
2.778 |
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| 400 |
2.255 |
2.203 |
2.229 |
2.100 |
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| 200 |
1.279 |
1.256 |
1.268 |
1.139 |
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| 100 |
0.659 |
0.655 |
0.657 |
0.528 |
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| 50 |
0.438 |
0.475 |
0.457 |
0.328 |
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| 25 |
0.256 |
0.242 |
0.249 |
0.120 |
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| 12.5 |
0.184 |
0.172 |
0.178 |
0.049 |
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| 0 |
0.126 |
0.132 |
0.129 |
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货期
3-5 working days