产品描述

This Human CFI ELISA Kit was designed for the quantitative measurement of Human CFI protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .

别名

AHUS3 ELISA Kit; ARMD13 ELISA Kit; C3b INA ELISA Kit; C3b inactivator ELISA Kit; C3B/C4B inactivator ELISA Kit; C3BINA ELISA Kit; CFAI_HUMAN ELISA Kit; Cfi ELISA Kit; Complement component I ELISA Kit; Complement control protein factor I ELISA Kit; Complement factor I ELISA Kit; Complement factor I heavy chain ELISA Kit; Complement factor I light chain ELISA Kit; F1 ELISA Kit; factor I ELISA Kit; FactorI ELISA Kit; FI ELISA Kit; I factor ELISA Kit; IF ELISA Kit; KAF ELISA Kit; Konglutinogen activating factor ELISA Kit; Light chain of factor I ELISA Kit; OTTHUMP00000219728 ELISA Kit; OTTHUMP00000221928 ELISA Kit

缩写

CFI

Uniprot No

P05156

灵敏度

1.56 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		92
		Range %		89-94
1:400		Average %		101
		Range %		98-103
1:800		Average %		92
		Range %		89-96
1:1600		Average %		97
		Range %		94-102

回收率

The recovery of human CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		96		92-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 400 2.780 2.766 2.773 2.635 200 2.288 2.311 2.300 2.162 100 1.743 1.684 1.714 1.576 50 1.114 1.101 1.108 0.970 25 0.622 0.652 0.637 0.499 12.5 0.345 0.336 0.341 0.203 6.25 0.237 0.224 0.231 0.093 0 0.140 0.136 0.138

货期

3-5 working days