产品描述
APOE全称为载脂蛋白E(Apolipoprotein E)。它是胆固醇和三酰甘油等脂质分子在体内运输的主要调节蛋白之一。
APOE有三种主要的基因型:APOEε2、APOEε3和APOEε4,它们的不同组合会影响人体对脂质代谢的调节能力。APOE在多种生理过程中都起到重要的作用,例如参与神经元的生长和修复、调节免疫反应等。
华美生物所提供的Human apolipoprotein E (Apo-E) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的APOE,其灵敏度为3.9 ng/mL,检测范围为15.62 ng/mL-1000 ng/mL。
别名
AD2 ELISA Kit; Apo-E ELISA Kit; APOE ELISA Kit; APOE_HUMAN ELISA Kit; APOEA ELISA Kit; Apolipoprotein E ELISA Kit; Apolipoprotein E3 ELISA Kit; ApolipoproteinE ELISA Kit; Apoprotein ELISA Kit; LDLCQ5 ELISA Kit; LPG ELISA Kit
缩写
APOE
Uniprot No
P02649
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
91 |
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| Range % |
86-95 |
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| 1:200 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:400 |
Average % |
91 |
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| Range % |
85-97 |
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| 1:800 |
Average % |
97 |
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| Range % |
91-103 |
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回收率
| The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.088 |
0.090 |
0.089 |
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| 500 |
0.135 |
0.142 |
0.139 |
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| 250 |
0.227 |
0.237 |
0.232 |
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| 125 |
0.324 |
0.341 |
0.333 |
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| 62.5 |
0.583 |
0.598 |
0.591 |
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| 31.25 |
0.847 |
0.864 |
0.856 |
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| 15.62 |
1.228 |
1.235 |
1.232 |
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| 0 |
2.155 |
2.199 |
2.177 |
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货期
3-5 working days