产品描述
α2-巨球蛋白(α2-MG)是一种大分子蛋白质,在人体中起着重要作用。它参与多种生理过程的调节,例如炎症、凝血和组织修复。 α2-MG 还充当血液循环中许多其他蛋白质和脂质的载体蛋白。它还具有抗炎特性,这使其成为药物开发的一个有吸引力的目标。 A2M 是科学文献中 α2-巨球蛋白的缩写。
华美生物所提供的Human α2 macroglobulin,α2 MG ELISA Kit属于
ELISA检测试剂盒,采用竞争法定量检测人血清、血浆、组织匀浆样本中的A2M,其灵敏度为1.25 μg/mL,检测范围为1.25 μg/mL-20 μg/mL。
别名
A2m ELISA Kit; A2MG_HUMAN ELISA Kit; Alpha 2 M ELISA Kit; Alpha 2M ELISA Kit; Alpha-2-M ELISA Kit; Alpha-2-macroglobulin ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 ELISA Kit; CPAMD5 ELISA Kit; DKFZp779B086 ELISA Kit; FWP007 ELISA Kit; S863 7 ELISA Kit
缩写
A2M
Uniprot No
P01023
灵敏度
1.25 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:200 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:400 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:800 |
Average % |
92 |
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| Range % |
87-96 |
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回收率
| The recovery of human α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-98 |
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| EDTA plasma (n=4) |
96 |
92-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.185 |
0.187 |
0.186 |
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| 10 |
0.360 |
0.365 |
0.363 |
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| 5 |
0.635 |
0.642 |
0.639 |
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| 2.5 |
1.163 |
1.172 |
1.168 |
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| 1.25 |
1.995 |
2.013 |
2.004 |
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| 0 |
2.849 |
2.924 |
2.887 |
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货期
3-5 working days