产品描述

The product CSB-E11178r is a sandwich ELISA kit developed to measure concentrations of rat sCD14 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of sCD14 in samples. The color intensity is measured at 450 nm via a microplate reader.

CD14 is a GPI-anchored receptor expressed by macrophages (MΦ) and neutrophils, facilitating pro- and anti-inflammatory cytokine production in response to various DAMPs. It functions in the TLR4-MD2 complex to initiate and activate proinflammatory signaling events in response to gram-negative bacteria through recognition of lipopolysaccharide (LPS). It plays a critical role in the phagocytic clearance of apoptotic cells and in the reactivation and immune recognition of microbial cell wall components of Gram-positive and Gram-negative bacteria. CD14 has also been reported to be upregulated during renal ischemia-reperfusion injury and in tubular epithelial cells of the kidney after unilateral ureteral obstruction.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 1800 2.530 2.401 2.466 2.283 900 2.066 1.986 2.026 1.843 450 1.311 1.356 1.334 1.151 225 0.759 0.779 0.769 0.586 112.5 0.456 0.478 0.467 0.284 56 0.352 0.344 0.348 0.165 28 0.271 0.263 0.267 0.084 0 0.181 0.185 0.183

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