产品描述
This Human CFP ELISA Kit was designed for the quantitative measurement of Human CFP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
别名
BFD ELISA Kit; CFP ELISA Kit; Complement factor P ELISA Kit; Complement factor properdin ELISA Kit; Factor P ELISA Kit; PFC ELISA Kit; PFD ELISA Kit; PROP_HUMAN ELISA Kit; Properdin ELISA Kit; Properdin P factor complement ELISA Kit
缩写
CFP
Uniprot No
P27918
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:100 | Average % | 95 | |
| Range % | 90-100 | |
| 1:200 | Average % | 106 | |
| Range % | 101-110 | |
| 1:400 | Average % | 98 | |
| Range % | 92-103 | |
| 1:800 | Average % | 90 | |
| Range % | 85-97 | |
回收率
| The recovery of human CFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 84 | 80-90 | |
| EDTA plasma (n=4) | 93 | 89-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.378 | 2.224 | 2.301 | 2.200 | | | 400 | 1.754 | 1.852 | 1.803 | 1.702 | | | 200 | 1.088 | 1.124 | 1.106 | 1.005 | | | 100 | 0.687 | 0.642 | 0.665 | 0.564 | | | 50 | 0.364 | 0.383 | 0.374 | 0.273 | | | 25 | 0.245 | 0.233 | 0.239 | 0.138 | | | 12.5 | 0.171 | 0.162 | 0.167 | 0.066 | | | 0 | 0.098 | 0.104 | 0.101 | | |
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货期
3-5 working days