产品描述

This Human ANGPTL7 ELISA Kit was designed for the quantitative measurement of Human ANGPTL7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Angiopoietin like 7 ELISA Kit; Angiopoietin like factor (CDT6) ELISA Kit; Angiopoietin like factor ELISA Kit; Angiopoietin related protein 7 ELISA Kit; Angiopoietin-like factor ELISA Kit; Angiopoietin-like protein 7 ELISA Kit; Angiopoietin-related protein 7 ELISA Kit; ANGL7_HUMAN ELISA Kit; ANGPTL7 ELISA Kit; AngX ELISA Kit; CDT6 ELISA Kit; Cornea derived transcript 6 protein ELISA Kit; Cornea-derived transcript 6 protein ELISA Kit; dJ647M16.1 ELISA Kit; OTTHUMP00000001986 ELISA Kit; RP4-647M16.2 ELISA Kit

缩写

ANGPTL7

Uniprot No

O43827

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		99
		Range %		92-104
1:2		Average %		87
		Range %		82-93
1:4		Average %		92
		Range %		87-98
1:8		Average %		101
		Range %		98-105

回收率

The recovery of human ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		92-106
EDTA plasma (n=4)		95		89-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 1.942 2.042 1.992 1.881 10 1.239 1.149 1.194 1.083 5 0.759 0.719 0.739 0.628 2.5 0.453 0.464 0.459 0.348 1.25 0.311 0.321 0.316 0.205 0.625 0.212 0.223 0.218 0.107 0.312 0.161 0.165 0.163 0.052 0 0.110 0.111 0.111

货期

3-5 working days