产品描述
CCL22,也称为小细胞肺癌分泌蛋白-1(MDC),是C-C趋化因子家族中的一种趋化因子。它主要由树突状细胞和肿瘤细胞等产生,并且能够吸引CCR4受体表达的细胞,如调节性T细胞和Th2细胞等。CCL22在多种疾病中发挥着重要作用,如过敏性疾病、自身免疫性疾病、肿瘤等。
华美生物所提供的Human Macrophage-Derived Chemokine (MDC/CCL22) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的CCL22,其灵敏度为10.324 pg/mL,检测范围为15.625 pg/mL-1000 pg/mL。
别名
A 152E5.1 ELISA Kit; ABCD 1 ELISA Kit; ABCD1 ELISA Kit; C C motif chemokine 22 ELISA Kit; CC chemokine STCP 1 ELISA Kit; CC chemokine STCP-1 ELISA Kit; ccl 22 ELISA Kit; Ccl22 ELISA Kit; CCL22_HUMAN ELISA Kit; Chemokine (C C motif) ligand 22 ELISA Kit; DC/B CK ELISA Kit; DCBCK ELISA Kit; Macrophage-derived chemokine ELISA Kit; MDC ELISA Kit; MDC(1-69) ELISA Kit; MDC(7-69) ELISA Kit; MGC34554 ELISA Kit; SCYA22 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 22 ELISA Kit; Small inducible cytokine subfamily A; member 22 ELISA Kit; Small-inducible cytokine A22 ELISA Kit; STCP 1 ELISA Kit; STCP1 ELISA Kit; Stimulated T cell chemotactic protein 1 ELISA Kit; Stimulated T-cell chemotactic protein 1 ELISA Kit
缩写
CCL22
Uniprot No
O00626
灵敏度
10.324 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-101 |
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| 1:4 |
Average % |
100 |
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| Range % |
94-112 |
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| 1:8 |
Average % |
93 |
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| Range % |
85-98 |
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回收率
| The recovery of human MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
80-93 |
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| EDTA plasma (n=4) |
89 |
85-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.534 |
2.421 |
2.478 |
2.349 |
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| 500 |
1.956 |
1.766 |
1.861 |
1.732 |
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| 250 |
1.147 |
1.087 |
1.117 |
0.988 |
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| 125 |
0.602 |
0.611 |
0.607 |
0.478 |
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| 62.5 |
0.341 |
0.331 |
0.336 |
0.207 |
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| 31.25 |
0.245 |
0.262 |
0.254 |
0.125 |
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| 15.625 |
0.198 |
0.201 |
0.200 |
0.071 |
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| 0 |
0.134 |
0.124 |
0.129 |
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货期
3-5 working days