产品描述

This Human CRTAC1 ELISA Kit was designed for the quantitative measurement of Human CRTAC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.68 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.

别名

68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit

缩写

CRTAC1

Uniprot No

Q9NQ79

灵敏度

1.17 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		91
		Range %		87-96
1:10		Average %		97
		Range %		93-101
1:20		Average %		102
		Range %		99-103
1:40		Average %		88
		Range %		86-91

回收率

The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		86-93
EDTA plasma (n=4)		102		98-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 300 2.522 2.735 2.629 2.444 150 2.043 2.102 2.073 1.888 75 1.317 1.315 1.316 1.131 37.5 0.875 0.857 0.866 0.681 18.75 0.470 0.445 0.458 0.273 9.37 0.366 0.380 0.373 0.188 4.68 0.254 0.252 0.253 0.068 0 0.186 0.184 0.185

货期

3-5 working days