产品描述
This Mouse ACV-B ELISA Kit was designed for the quantitative measurement of Mouse ACV-B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
ACV-B
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 92-105 | |
| 1:2 | Average % | 94 | |
| Range % | 85-100 | |
| 1:4 | Average % | 91 | |
| Range % | 86-98 | |
| 1:8 | Average % | 96 | |
| Range % | 91-104 | |
回收率
| The recovery of mouse ACV-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 90-95 | |
| EDTA plasma (n=4) | 95 | 92-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.278 | 2.252 | 2.265 | 2.179 | | | 200 | 2.132 | 2.015 | 2.074 | 1.988 | | | 100 | 1.612 | 1.641 | 1.627 | 1.541 | | | 50 | 0.987 | 0.933 | 0.960 | 0.874 | | | 25 | 0.541 | 0.522 | 0.532 | 0.446 | | | 12.5 | 0.278 | 0.285 | 0.282 | 0.196 | | | 6.25 | 0.184 | 0.194 | 0.189 | 0.103 | | | 0 | 0.085 | 0.087 | 0.086 | | |
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货期
3-5 working days