产品描述
This Mouse AVP ELISA Kit was designed for the quantitative measurement of Mouse AVP protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .
别名
ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
缩写
AVP
灵敏度
0.31 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-102 | |
| 1:2 | Average % | 89 | |
| Range % | 84-95 | |
| 1:4 | Average % | 87 | |
| Range % | 82-96 | |
| 1:8 | Average % | 96 | |
| Range % | 90-104 | |
回收率
| The recovery of mouse ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 83-94 | |
| EDTA plasma (n=4) | 102 | 98-107 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.543 | 2.578 | 2.561 | 2.373 | | | 40 | 1.820 | 1.846 | 1.833 | 1.645 | | | 20 | 1.172 | 1.198 | 1.185 | 0.997 | | | 10 | 0.782 | 0.795 | 0.789 | 0.601 | | | 5 | 0.447 | 0.468 | 0.458 | 0.270 | | | 2.5 | 0.323 | 0.339 | 0.331 | 0.143 | | | 1.25 | 0.291 | 0.270 | 0.281 | 0.093 | | | 0 | 0.187 | 0.189 | 0.188 | | |
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货期
3-5 working days