产品描述
This Rat CCL4 ELISA Kit was designed for the quantitative measurement of Rat CCL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4C-C motif chemokine 4 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Small-inducible cytokine A4 ELISA Kit
缩写
CCL4
Uniprot No
P50230
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 106 | |
| Range % | 101-112 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 92-104 | |
回收率
| The recovery of rat MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-94 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.564 | 2.476 | 2.520 | 2.375 | | | 100 | 2.174 | 2.014 | 2.094 | 1.949 | | | 50 | 1.418 | 1.372 | 1.395 | 1.250 | | | 25 | 0.917 | 0.973 | 0.945 | 0.800 | | | 12.5 | 0.555 | 0.589 | 0.572 | 0.427 | | | 6.25 | 0.394 | 0.417 | 0.406 | 0.261 | | | 3.12 | 0.289 | 0.273 | 0.281 | 0.136 | | | 0 | 0.148 | 0.141 | 0.145 | | |
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货期
3-5 working days