产品描述
The Rat apolipoprotein H (Apo-H) ELISA Kit is the reliable and accurate solution for detecting and quantifying Beta-2-glycoprotein 1, also known as Apolipoprotein H (Apo-H) or Beta-2-glycoprotein I (B2GPI), in rat serum, plasma, and tissue homogenates.
With its detection range of 28 ng/mL-1800 ng/mL and a sensitivity of 7 ng/mL, this ELISA kit allows for precise measurements of Apo-H levels in a variety of sample types. The assay time is 1-5 hours, and the sample volume required is only 50-100 µL, making it a convenient and efficient choice for researchers in the cardiovascular field.
The Rat apolipoprotein H (Apo-H) ELISA Kit follows a sandwich assay principle, where the sample is captured by an immobilized antibody specific to rat Apo-H and then detected by a biotinylated antibody. The amount of detected signal is proportional to the concentration of Apo-H in the sample, which is measured at a detection wavelength of 450 nm. The high sensitivity, wide detection range, and easy-to-use protocol make this kit an ideal choice for researchers in the area.
别名
ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
缩写
APOH
Uniprot No
P26644
灵敏度
7 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:200 | Average % | 91 | |
| Range % | 86-95 | |
| 1:400 | Average % | 102 | |
| Range % | 97-107 | |
| 1:800 | Average % | 91 | |
| Range % | 85-97 | |
| 1:1600 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 107 | 101-110 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1800 | 2.655 | 2.532 | 2.594 | 2.464 | | | 900 | 2.164 | 2.117 | 2.141 | 2.011 | | | 450 | 1.597 | 1.544 | 1.571 | 1.441 | | | 225 | 0.845 | 0.828 | 0.837 | 0.707 | | | 112 | 0.501 | 0.511 | 0.506 | 0.376 | | | 56 | 0.307 | 0.300 | 0.304 | 0.174 | | | 28 | 0.234 | 0.222 | 0.228 | 0.098 | | | 0 | 0.131 | 0.129 | 0.130 | | |
|
货期
3-5 working days
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