产品描述
This human CTSK ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CTSK in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of CTSK bound in the initial step.
CTSK is a cysteine proteinase that is primarily expressed in osteoclasts and mainly functions to mediate bone resorption. CTSK cleaves key bone matrix proteins like type I collagen and plays an important role in degrading the organic phase of bone during bone resorption. Mutations in the CTSK gene lead to an autosomal recessive osteosclerotic skeletal disorder-pycnodysostosis. CTSK is involved in cartilage matrix degradation and osteoarthritis (OA) because it also degrades type II collagen, the predominant matrix protein of cartilage.
别名
Cathepsin K ELISA Kit; Cathepsin O ELISA Kit; Cathepsin O1 ELISA Kit; Cathepsin O2 ELISA Kit; Cathepsin X ELISA Kit; CATK_HUMAN ELISA Kit; CTS02 ELISA Kit; Ctsk ELISA Kit; CTSO ELISA Kit; CTSO1 ELISA Kit; CTSO2 ELISA Kit; MGC23107 ELISA Kit; PKND ELISA Kit; PYCD ELISA Kit
缩写
CTSK
Uniprot No
P43235
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-105 | |
| 1:2 | Average % | 92 | |
| Range % | 88-98 | |
| 1:4 | Average % | 85 | |
| Range % | 80-90 | |
| 1:8 | Average % | 95 | |
| Range % | 91-100 | |
回收率
| The recovery of human cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-102 | |
| EDTA plasma (n=4) | 95 | 88-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 1.886 | 1.822 | 1.854 | 1.735 | | | 250 | 1.568 | 1.489 | 1.529 | 1.410 | | | 125 | 1.213 | 1.201 | 1.207 | 1.088 | | | 62.5 | 0.976 | 0.939 | 0.958 | 0.839 | | | 31.2 | 0.652 | 0.624 | 0.638 | 0.519 | | | 15.6 | 0.348 | 0.325 | 0.337 | 0.218 | | | 7.8 | 0.206 | 0.199 | 0.203 | 0.084 | | | 0 | 0.121 | 0.117 | 0.119 | | |
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货期
3-5 working days
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