产品描述
CAT (Catalase) 是一种主要的抗氧化酶,可以降解过氧化氢分子,从而保护细胞免受过氧化氢对细胞膜、DNA等生物分子的氧化破坏。
华美生物所提供的Rat Catalase (CAT) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清样本中的CAT,其灵敏度为23.43 mIU/mL,检测范围为93.75 mIU/mL-6000 mIU/mL。
别名
Cat ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
缩写
CAT
Uniprot No
P04762
灵敏度
23.43 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:10 |
Average % |
94 |
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| Range % |
90-97 |
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| 1:20 |
Average % |
105 |
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| Range % |
98-109 |
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| 1:40 |
Average % |
88 |
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| Range % |
82-94 |
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回收率
| The recovery of rat CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
82-94 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 6000 |
2.703 |
2.602 |
2.653 |
2.537 |
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| 3000 |
1.962 |
1.861 |
1.912 |
1.796 |
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| 1500 |
1.218 |
1.197 |
1.208 |
1.092 |
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| 750 |
0.734 |
0.723 |
0.729 |
0.613 |
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| 375 |
0.387 |
0.398 |
0.393 |
0.277 |
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| 187.5 |
0.297 |
0.286 |
0.292 |
0.176 |
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| 93.75 |
0.188 |
0.184 |
0.186 |
0.070 |
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| 0 |
0.115 |
0.117 |
0.116 |
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货期
3-5 working days