产品描述
This Human ADM ELISA Kit was designed for the quantitative measurement of Human ADM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pg/mL-20 pg/mL and the sensitivity is 0.078 pg/mL.
别名
Adm ELISA Kit; ADM precursor ELISA Kit; ADML_HUMAN ELISA Kit; Adrenomedullin ELISA Kit; AM ELISA Kit; PAMP ELISA Kit; Preproadrenomedullin ELISA Kit; Proadrenomedullin N-20 terminal peptide ELISA Kit; Proadrenomedullin N20 terminal peptide ELISA Kit; ProAM N terminal 20 peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; ProAM N20 ELISA Kit; ProAM-N20 ELISA Kit; ProAMN20 ELISA Kit
缩写
ADM
Uniprot No
P35318
灵敏度
0.078 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 96 | |
| Range % | 90-104 | |
| 1:10 | Average % | 94 | |
| Range % | 86-98 | |
| 1:20 | Average % | 88 | |
| Range % | 80-95 | |
| 1:40 | Average % | 93 | |
| Range % | 88-100 | |
回收率
| The recovery of human ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-100 | |
| EDTA plasma (n=4) | 90 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.314 | 2.131 | 2.223 | 2.025 | | | 10 | 1.923 | 1.902 | 1.913 | 1.715 | | | 5 | 1.428 | 1.381 | 1.405 | 1.207 | | | 2.5 | 1.003 | 0.992 | 0.998 | 0.800 | | | 1.25 | 0.749 | 0.731 | 0.740 | 0.542 | | | 0.625 | 0.528 | 0.509 | 0.519 | 0.321 | | | 0.312 | 0.302 | 0.294 | 0.298 | 0.100 | | | 0 | 0.199 | 0.197 | 0.198 | | |
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货期
3-5 working days