产品描述
促肾上腺皮质激素(ACTH)是一种多功能的生物激素,可以在人体内引发一系列生理和行为反应。它是由脑垂体中的垂体前叶产生的,并通过血液循环传递到全身各个部位。ACTH有助于促进新陈代谢、影响机体对应激性物反应。
华美生物所提供的Rat adrenocorticotropic hormone (ACTH) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的ACTH,其灵敏度为华美生物所提供的1.25 pg/mL,检测范围为1.25 pg/mL-50 pg/mL。
缩写
ACTH
灵敏度
1.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<15% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
95 |
|
| Range % |
90-99 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
91-102 |
|
| 1:4 |
Average % |
86 |
|
| Range % |
82-94 |
|
| 1:8 |
Average % |
89 |
|
| Range % |
82-96 |
|
回收率
| The recovery of rat ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
86-96 |
|
| EDTA plasma (n=4) |
101 |
97-105 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
| 50 |
0.341 |
0.340 |
0.341 |
|
|
| 25 |
0.476 |
0.474 |
0.475 |
|
|
| 11.5 |
0.700 |
0.713 |
0.707 |
|
|
| 3.75 |
1.056 |
1.067 |
1.062 |
|
|
| 1.25 |
1.468 |
1.387 |
1.428 |
|
|
| 0 |
1.945 |
1.864 |
1.905 |
|
|
|
货期
3-5 working days