产品描述
This Human Ang-Ⅰ ELISA Kit was designed for the quantitative measurement of Human Ang-Ⅰ protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
angiotensin Ⅰ,Ang-Ⅰ,angiotensin 1,Ang-1
缩写
Ang-Ⅰ
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 100 | |
| Range % | 97-105 | |
| 1:2 | Average % | 98 | |
| Range % | 94-103 | |
| 1:4 | Average % | 105 | |
| Range % | 100-110 | |
| 1:8 | Average % | 108 | |
| Range % | 102-114 | |
回收率
| The recovery of human Ang-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-97 | |
| EDTA plasma (n=4) | 95 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.683 | 2.752 | 2.718 | 2.567 | | | 500 | 1.905 | 1.989 | 1.947 | 1.796 | | | 250 | 1.037 | 1.122 | 1.080 | 0.929 | | | 125 | 0.631 | 0.655 | 0.643 | 0.492 | | | 62.5 | 0.431 | 0.465 | 0.448 | 0.297 | | | 31.2 | 0.307 | 0.321 | 0.314 | 0.163 | | | 15.6 | 0.230 | 0.224 | 0.227 | 0.076 | | | 0 | 0.147 | 0.154 | 0.151 | | |
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货期
3-5 working days