产品描述

This Human CREB1 ELISA Kit was designed for the quantitative measurement of Human CREB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Active transcription factor CREB ELISA Kit; cAMP response element binding protein 1 ELISA Kit; cAMP response element binding protein ELISA Kit; cAMP responsive element binding protein 1 ELISA Kit; cAMP-responsive element-binding protein 1 ELISA Kit; CREB ELISA Kit; CREB-1 ELISA Kit; CREB1 ELISA Kit; CREB1_HUMAN ELISA Kit; Cyclic AMP-responsive element-binding protein 1 ELISA Kit; MGC9284 ELISA Kit; OTTHUMP00000163864 ELISA Kit; OTTHUMP00000163865 ELISA Kit; OTTHUMP00000206660 ELISA Kit; OTTHUMP00000206662 ELISA Kit; OTTHUMP00000206667 ELISA Kit; Transactivator protein ELISA Kit

缩写

CREB1

Uniprot No

P16220

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		92
		Range %		87-96
1:10		Average %		89
		Range %		85-94
1:20		Average %		95
		Range %		89-98
1:40		Average %		102
		Range %		98-105

回收率

The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		93-102
EDTA plasma (n=4)		86		80-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.157 2.254 2.206 2.104 10 1.715 1.812 1.764 1.662 5 1.267 1.284 1.276 1.174 2.5 0.857 0.863 0.860 0.758 1.25 0.523 0.532 0.528 0.426 0.625 0.343 0.351 0.347 0.245 0.312 0.206 0.213 0.210 0.108 0 0.103 0.101 0.102

货期

3-5 working days