产品描述

This Human ABL1 ELISA Kit was designed for the quantitative measurement of Human ABL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

Abelson murine leukemia viral oncogene homolog 1 ELISA Kit; Abelson tyrosine protein kinase 1 ELISA Kit; Abl 1 ELISA Kit; ABL ELISA Kit; ABL proto oncogene 1 non receptor tyrosine kinase ELISA Kit; ABL1 ELISA Kit; ABL1_HUMAN ELISA Kit; bcr/abl ELISA Kit; bcr/c abl oncogene protein ELISA Kit; c ABL ELISA Kit; c abl oncogene 1 non receptor tyrosine kinase ELISA Kit; c abl oncogene 1 receptor tyrosine kinase ELISA Kit; c ABL1 ELISA Kit; JTK7 ELISA Kit; p150 ELISA Kit; Proto oncogene tyrosine protein kinase ABL1 ELISA Kit; Proto-oncogene c-Abl ELISA Kit; Tyrosine-protein kinase ABL1 ELISA Kit; v abl Abelson murine leukemia viral oncogene homolog 1 ELISA Kit; v abl ELISA Kit

缩写

ABL1

Uniprot No

P00519

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ABL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		85-102
1:2		Average %		98
		Range %		95-114
1:4		Average %		93
		Range %		80-107
1:8		Average %		85
		Range %		80-89

回收率

The recovery of human ABL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-97
EDTA plasma (n=4)		87		80-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.287 2.312 2.300 2.110 800 1.563 1.656 1.610 1.420 400 1.025 1.054 1.040 0.850 200 0.644 0.655 0.650 0.460 100 0.448 0.474 0.461 0.271 50 0.311 0.325 0.318 0.128 25 0.289 0.288 0.289 0.099 0 0.189 0.190 0.190

货期

3-5 working days