产品描述
This Human Ang1-7 ELISA Kit was designed for the quantitative measurement of Human Ang1-7 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
缩写
Ang1-7
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 80-105 | |
| 1:2 | Average % | 96 | |
| Range % | 85-109 | |
| 1:4 | Average % | 105 | |
| Range % | 92-114 | |
| 1:8 | Average % | 95 | |
| Range % | 82-110 | |
回收率
| The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 82-110 | |
| EDTA plasma (n=4) | 99 | 90-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.667 | 2.684 | 2.676 | 2.525 | | | 250 | 1.934 | 1.939 | 1.937 | 1.786 | | | 125 | 1.123 | 1.119 | 1.121 | 0.970 | | | 62.5 | 0.690 | 0.674 | 0.682 | 0.531 | | | 31.2 | 0.462 | 0.471 | 0.467 | 0.316 | | | 15.6 | 0.316 | 0.306 | 0.311 | 0.160 | | | 7.8 | 0.236 | 0.225 | 0.231 | 0.080 | | | 0 | 0.152 | 0.150 | 0.151 | | |
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货期
3-5 working days