产品描述
This Mouse CTSK ELISA Kit was designed for the quantitative measurement of Mouse CTSK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
缩写
CTSK
Uniprot No
P55097
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-98 | |
| 1:2 | Average % | 99 | |
| Range % | 94-103 | |
| 1:4 | Average % | 84 | |
| Range % | 80-88 | |
| 1:8 | Average % | 105 | |
| Range % | 100-112 | |
回收率
| The recovery of mouse cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-99 | |
| EDTA plasma (n=4) | 90 | 85-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.813 | 2.687 | 2.750 | 2.602 | | | 500 | 2.198 | 2.256 | 2.227 | 2.079 | | | 250 | 1.578 | 1.667 | 1.623 | 1.475 | | | 125 | 0.941 | 0.925 | 0.933 | 0.785 | | | 62.5 | 0.573 | 0.559 | 0.566 | 0.418 | | | 31.2 | 0.321 | 0.349 | 0.335 | 0.187 | | | 15.6 | 0.246 | 0.235 | 0.241 | 0.093 | | | 0 | 0.151 | 0.144 | 0.148 | | |
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货期
3-5 working days