产品描述

This rat GM-CSF ELISA Kit is suitable for qualitatively determining GM-CSF concentrations in multiple biological fluids, including rat serum, plasma, and tissue homogenates in vitro. GM-CSF, also called CSF2, is a growth factor that induces the differentiation and proliferation of myeloid progenitors in the bone marrow. It also exerts a cytokine effect in chronic inflammatory diseases by stimulating the activation and migration of myeloid cells to inflammation sites, promoting the survival of target cells, and stimulating the renewal of effector granulocytes and macrophages. GM-CSF-CSFR2 signaling activates PI3K and JAK/STAT-Bcl-2 signaling pathways thus inducing cell differentiation and promoting cell survival. Imbalanced GM-CSF production or dysregulated GM-CSF signaling may cause harmful inflammatory conditions such as multiple sclerosis (MS) and rheumatoid arthritis (RA).

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat GM-CSF in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-rat GM-CSF antibody. Biotin-labeled GM-CSF antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the GM-CSF in the rat samples. GM-CSF binds to the biotinylated anti-rat GM-CSF monoclonal antibody. And the biotin on the biotinylated anti-rat GM-CSF monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of GM-CSF in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.762 2.856 2.809 2.633 500 2.190 2.223 2.207 2.031 250 1.411 1.435 1.423 1.247 125 0.845 0.856 0.851 0.675 62.5 0.556 0.561 0.559 0.383 31.2 0.378 0.382 0.380 0.204 15.6 0.254 0.269 0.262 0.086 0 0.175 0.176 0.176

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