产品描述

The ELISA Kit is designed for quantitatively measuring human ADAM9 levels in samples, including serum, plasma, tissue homogenates, cell culture supernates, or ascitic fluid. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM9 in samples. The color intensity is measured at 450 nm via a microplate reader.

ADAM9 is a metzincin cell-surface protease involved in several biological processes such as myogenesis, fertilization, cell migration, inflammatory response, proliferation, and cell-cell interactions. It is involved in inflammation, degenerative diseases, and cancer. In inflammation, ADAM9 contributes to monocyte fusion, mediating the conversion of monocytes-macrophages to multinucleated giant cells (MGCs) in response to foreign bodies or bacteria. The generated granulomatous lesions help to isolate the pathogens and also enhance phagocytotic activity. ADAM9 has been found over-expressed in several solid tumors such as glioma, melanoma, prostate cancer, pancreatic ductal adenocarcinoma, as well as gastric, breast, lung, and liver cancers. Upregulation of ADAM9 is associated with tumor aggressiveness and poor prognosis.

别名

ADAM 9 ELISA Kit; ADAM metallopeptidase domain 9 ELISA Kit; Adam9 ELISA Kit; ADAM9_HUMAN ELISA Kit; Cellular disintegrin-related protein ELISA Kit; Cone rod dystrophy 9 ELISA Kit; CORD9 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 9 ELISA Kit; MCMP ELISA Kit; MDC9 ELISA Kit; Meltrin-gamma ELISA Kit; Metalloprotease/disintegrin/cysteine-rich protein 9 ELISA Kit; Mltng ELISA Kit; Myeloma cell metalloproteinase ELISA Kit

缩写

ADAM9

Uniprot No

Q13443

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		9-101
1:2		Average %		87
		Range %		84-92
1:4		Average %		95
		Range %		92-97
1:8		Average %		93
		Range %		89-99

回收率

The recovery of human ADAM9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-93
EDTA plasma (n=4)		93		89-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 2.657 2.557 2.607 2.445 2000 1.924 1.824 1.874 1.712 1000 1.126 1.116 1.121 0.959 500 0.655 0.645 0.650 0.488 250 0.380 0.370 0.375 0.213 125 0.271 0.261 0.266 0.104 62.5 0.215 0.212 0.214 0.052 0 0.163 0.161 0.162

货期

3-5 working days