产品描述
CCL22是一种趋化因子,也称为室内过敏原-4(MDC-4)。它主要由多种免疫细胞产生,如巨噬细胞、树突状细胞和内皮细胞等,能够通过结合CCR4受体引导CCR4受体表达的细胞向其所在的组织移动。CCL22在炎症、自身免疫和肿瘤免疫等过程中发挥着重要作用,因此被广泛研究作为治疗疾病的潜在靶点。
华美生物所提供的Mouse Macrophage-Derived Chemokine,MDC ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆、细胞培养上清、细胞裂解液样本中的CCL22,其灵敏度为23.672 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
缩写
CCL22
Uniprot No
O88430
灵敏度
23.672 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
83-92 |
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| 1:2 |
Average % |
100 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
94 |
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| Range % |
81-98 |
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| 1:8 |
Average % |
99 |
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| Range % |
90-103 |
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回收率
| The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
89-103 |
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| EDTA plasma (n=4) |
93 |
84-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.888 |
2.812 |
2.850 |
2.683 |
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| 1000 |
2.589 |
2.513 |
2.551 |
2.384 |
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| 500 |
2.110 |
2.168 |
2.139 |
1.972 |
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| 250 |
1.463 |
1.446 |
1.455 |
1.288 |
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| 125 |
0.810 |
0.827 |
0.819 |
0.652 |
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| 62.5 |
0.537 |
0.554 |
0.546 |
0.379 |
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| 31.25 |
0.320 |
0.311 |
0.316 |
0.149 |
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| 0 |
0.169 |
0.165 |
0.167 |
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货期
3-5 working days