产品描述

The ELISA Kit is designed for quantitatively measuring human AGR2 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of AGR2 in samples. The color intensity is measured at 450 nm via a microplate reader.

AGR2 is a dimeric protein disulfide isomerase family member that plays a vital role in oxidative protein folding in the endoplasmic reticulum (ER). It acts as a chaperone that mediates the formation of disulfide bonds, catalyzes the cysteine-based redox reactions, and assists the quality control of proteins. AGR2 is involved in several signal transduction pathways that are essential for cell survival. Accumulating evidence has shown that AGR2 is a pro-oncogenic protein implicated in tumorigenesis and cancer progression. Overexpression of AGR2 has been reported in multiple solid human tumors, including breast, prostate, ovarian, lung, esophageal, gastric, colorectal, and pancreatic cancers, and has been associated with adenocarcinoma growth, cell metastasis, and drug resistance.

别名

AG 2 ELISA Kit; AG 2 protein ELISA Kit; AG-2 ELISA Kit; AG2 ELISA Kit; AG2 protein ELISA Kit; AGR 2 ELISA Kit; AGR2 ELISA Kit; AGR2_HUMAN ELISA Kit; Anterior gradient 2 homolog (Xenopus laevis) ELISA Kit; anterior gradient 2 homolog ELISA Kit; Anterior gradient 2; protein disulphide isomerase family member ELISA Kit; Anterior gradient 2; Xenopus; homolog of ELISA Kit; Anterior gradient homolog 2 ELISA Kit; Anterior gradient protein 2 homolog ELISA Kit; Epididymis secretory protein Li 116 ELISA Kit; GOB 4 ELISA Kit; GOB4 ELISA Kit; HAG 2 ELISA Kit; hAG-2 ELISA Kit; hAG2 ELISA Kit; HAG2/R ELISA Kit; HEL S 116 ELISA Kit; HPC 8 ELISA Kit; hPC8 ELISA Kit; PDIA17 ELISA Kit; Protein disulfide isomerase family A member 17 ELISA Kit; secreted cement gland homolog ELISA Kit; Secreted cement gland protein XAG 2 homolog ELISA Kit; Secreted cement gland protein XAG-2 homolog ELISA Kit; Secreted cement gland protein XAG2 homolog ELISA Kit; XAG 2 ELISA Kit; XAG2 ELISA Kit

缩写

AGR2

Uniprot No

O95994

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AGR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-104
1:2		Average %		101
		Range %		95-110
1:4		Average %		92
		Range %		88-96
1:8		Average %		85
		Range %		80-90

回收率

The recovery of human AGR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-100
EDTA plasma (n=4)		87		81-91

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.501 2.427 2.464 2.296 5 1.943 2.075 2.009 1.841 2.5 1.427 1.462 1.445 1.277 1.25 0.901 0.945 0.923 0.755 0.625 0.610 0.584 0.597 0.429 0.312 0.411 0.430 0.421 0.253 0.156 0.291 0.304 0.298 0.130 0 0.165 0.171 0.168

货期

3-5 working days