产品描述
This Pig ANGPT2 ELISA Kit was designed for the quantitative measurement of Pig ANGPT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.235 ng/mL.
别名
ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
缩写
ANGPT2
Uniprot No
Q9BDY7
灵敏度
0.235 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 83-89 | |
| 1:2 | Average % | 99 | |
| Range % | 96-102 | |
| 1:4 | Average % | 87 | |
| Range % | 84-90 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-92 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 60 | 2.509 | 2.596 | 2.553 | 2.401 | | | 30 | 1.749 | 1.768 | 1.759 | 1.607 | | | 15 | 1.004 | 1.068 | 1.036 | 0.884 | | | 7.5 | 0.663 | 0.642 | 0.653 | 0.501 | | | 3.75 | 0.390 | 0.378 | 0.384 | 0.232 | | | 1.88 | 0.298 | 0.290 | 0.294 | 0.142 | | | 0.94 | 0.224 | 0.239 | 0.232 | 0.080 | | | 0 | 0.151 | 0.153 | 0.152 | | |
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货期
3-5 working days