产品描述
The product CSB-E09171h is a sandwich ELISA kit developed to measure concentrations of human CTLA4 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CTLA4 in samples. The color intensity is measured at 450 nm via a microplate reader.
CTLA4 is a membrane glycoprotein constitutively expressed on regulatory T (Treg) cells and upregulated on activated T cells. It is involved in the repression of T cell proliferation, cell cycle progression, and cytokine production. CTLA-4 inhibits T cell activation by various suppressive functions including competition with CD28, modulation of the inhibitory function of Treg cells, such as transendocytosis, and the control of adhesion and motility. It is a critical modulator of T cell homeostasis and self-tolerance. Studies have CTLA4 can inhibit cell cycle progression in T-cells by suppressing the generation of cyclin D3 and cyclin-dependent kinases. Human CTLA-4 insufficiency is caused by heterozygous germline mutations in CTLA4 and is characterized by a complex immune dysregulation syndrome.
别名
CTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152
缩写
CTLA4
Uniprot No
P16410
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
99 |
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| Range % |
96-102 |
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| 1:2 |
Average % |
87 |
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| Range % |
84-93 |
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| 1:4 |
Average % |
111 |
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| Range % |
107-115 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-108 |
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回收率
| The recovery of human CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-89 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 8000 |
2.303 |
2.398 |
2.351 |
2.249 |
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| 4000 |
1.420 |
1.468 |
1.444 |
1.342 |
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| 2000 |
0.745 |
0.704 |
0.725 |
0.623 |
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| 1000 |
0.423 |
0.409 |
0.416 |
0.314 |
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| 500 |
0.285 |
0.298 |
0.292 |
0.190 |
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| 250 |
0.222 |
0.232 |
0.227 |
0.125 |
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| 125 |
0.161 |
0.168 |
0.165 |
0.063 |
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| 0 |
0.103 |
0.101 |
0.102 |
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货期
3-5 working days
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